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Please use this identifier to cite or link to this item: http://repository.iitr.ac.in/handle/123456789/13324
Title: Production of novel alkali-thermo-tolerant cellulase-poor xylanases from Coprinopsis cinerea HK-1 NFCCI-2032
Authors: Kaur H.
Dutt, Dharm
Tyagi C.H.
Published in: BioResources
Abstract: Culture conditions of a newly isolated basidiomycetous strain were optimized for the enhanced production of extracellular alkali-thermo-tolerant cellulase-poor xylanase using wheat bran as the sole carbon source under solid state fermentation (SSF). SEM and ITS sequencing confirmed it as Coprinopsis cinerea HK-1 NFCCI-2032. Among various inexpensive agro-residues, wheat bran (carbon source) came up as the most potent enzyme inducer under SSF, and resulted in 54% higher xylanase activity compared to that in submerged fermentation mode. The strain grew well even at 47 °C. The highest xylanase (695.8 IU/mL) titer was recorded at a substrate:moisture ratio of 1:3 after 7 days of incubation at 37 °C at pH 6.4 along with 0.541 IU/mL of poorly associated cellulase activity. The xylanase exhibited remarkable stability and retained 50% of its activity at pH 8.0 on incubation at 55 °C for 15 min and 78, 43, and 23% of its activity at temperatures 65, 75 and 85 °C, respectively, demonstrating an approximately 50% alkali-thermo-tolerant nature, which is suitable for biobleaching.
Citation: BioResources (2011), 6(2): 1376-1391
URI: http://repository.iitr.ac.in/handle/123456789/13324
Issue Date: 2011
Keywords: Alkali-thermo-tolerant
Cellulase-poor xylanases
Coprinopsis cinerea HK-1 NFCCI-2032
ITS sequencing
Solid-state fermentation
ISSN: 19302126
Author Scopus IDs: 55553726762
7005982560
6602705260
Author Affiliations: Kaur, H., Department of Paper Technology, Indian Institute of Technology Roorkee, Saharanpur campus, Saharanpur 247 001, India
Dutt, D., Department of Paper Technology, Indian Institute of Technology Roorkee, Saharanpur campus, Saharanpur 247 001, India
Tyagi, C.H., Department of Paper Technology, Indian Institute of Technology Roorkee, Saharanpur campus, Saharanpur 247 001, India
Corresponding Author: Kaur, H.; Department of Paper Technology, Indian Institute of Technology Roorkee, Saharanpur campus, Saharanpur 247 001, India; email: harjeet.iitr@gmail.com
Appears in Collections:Journal Publications [PT]

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