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Please use this identifier to cite or link to this item: http://repository.iitr.ac.in/handle/123456789/13323
Title: Production of high level of cellulase-poor xylanases by wild strains of white-rot fungus Coprinellus disseminatus in solid-state fermentation
Authors: Singh S.
Tyagi C.H.
Dutt, Dharm
Upadhyaya J.S.
Published in: New Biotechnology
Abstract: The production, optimisation and partial characterisation of xylanases from newly isolated wild strains of Coprinellus disseminatus was performed in solid-state fermentation. Strains SH-1 and SH-2 showed high xylanase (727.78 and 227.99 IU/mL) with very low CMCase (0.925 and 0.660 IU/mL) and laccase (0.640 and 0.742 U/mL) activities at incubation time seven days, 37°C and initial pH 6.4, using yeast extract as nitrogen source and cheap substrate (wheat bran), which increased the cost effectiveness of the process. Crude cellulase-poor xylanases obtained from test strains showed maximum activities at 55°C and pH 6.4 and retained 32.64 (SH-1) and 35.03% (SH-2) activity at pH 8 and 43.01 (SH-1) and 25.00% (SH-2) activity at 65°C. As test strains produced high level of cellulase-poor xylanases, which were active over a wide range of temperature and pH, these enzymes might be used as pulp biobleaching agents. © 2009 Elsevier B.V. All rights reserved.
Citation: New Biotechnology (2009), 26(43894): 165-170
URI: https://doi.org/10.1016/j.nbt.2009.09.004
http://repository.iitr.ac.in/handle/123456789/13323
Issue Date: 2009
Publisher: Elsevier
ISSN: 18716784
Author Scopus IDs: 7406402372
6602705260
7005982560
8679068400
Author Affiliations: Singh, S., Department of Paper Technology, Indian Institute of Technology Roorkee, Saharanpur Campus, Roorkee, India
Tyagi, C.H., Department of Paper Technology, Indian Institute of Technology Roorkee, Saharanpur Campus, Roorkee, India
Dutt, D., Department of Paper Technology, Indian Institute of Technology Roorkee, Saharanpur Campus, Roorkee, India
Upadhyaya, J.S., Department of Paper Technology, Indian Institute of Technology Roorkee, Saharanpur Campus, Roorkee, India
Corresponding Author: Singh, S.; Department of Paper Technology, Indian Institute of Technology Roorkee, Saharanpur Campus, Roorkee, India; email: shalinisingh.iit@gmail.com
Appears in Collections:Journal Publications [PT]

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