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Please use this identifier to cite or link to this item: http://repository.iitr.ac.in/handle/123456789/13322
Title: Production of crude enzyme from Aspergillus nidulans AKB-25 using black gram residue as the substrate and its industrial applications
Authors: Kumar A.
Dutt, Dharm
Gautam A.
Published in: Journal of Genetic Engineering and Biotechnology
Abstract: The production of crop residues in India is estimated to be about 500–550 million tons annually. It is estimated that about 93 million tons of crop residues is burnt annually which is not only wastage of valuable biomass resources but pollution of the environment with the production of green house gases also. Among different low cost crop residues, black gram residue as the substrate produced maximal endoglucanase, FPase, and β-glucosidase activities from Aspergillus nidulans AKB-25 under solid-state fermentation. During optimisation of cultural parameters A. nidulans AKB-25 produced maximal endoglucanase (152.14 IU/gds), FPase (3.42 FPU/gds) and xylanase (2441.03 IU/gds) activities. The crude enzyme was found effective for the saccharification of pearl millet stover and bio-deinking of mixed office waste paper. The crude enzyme from A. nidulans AKB-25 produced maximum fermentable sugars of 546.91 mg/g from alkali-pretreated pearl millet stover by saccharification process at a dose of 15 FPU/g of substrate. Pulp brightness and deinking efficiency of mixed office waste paper improved by 4.6% and 25.01% respectively and mitigated dirt counts by 74.70% after bio-deinking. Physical strength properties like burst index, tensile index and double fold number were also improved during bio-deinking of mixed office waste paper. © 2016
Citation: Journal of Genetic Engineering and Biotechnology (2016), 14(1): 107-118
URI: https://doi.org/10.1016/j.jgeb.2016.06.004
http://repository.iitr.ac.in/handle/123456789/13322
Issue Date: 2016
Publisher: Academy of Scientific Research and Technology
Keywords: Aspergillus nidulans
Bio-deinking
Biomass-degrading enzymes
Black gram residue
Pearl millet stover
Saccharification
ISSN: 1687157X
Author Scopus IDs: 57206266862
7005982560
57198173170
Author Affiliations: Kumar, A., Department of Paper Technology, Indian Institute of Technology Roorkee, Saharanpur Campus, Saharanpur, 247 001, India
Dutt, D., Department of Paper Technology, Indian Institute of Technology Roorkee, Saharanpur Campus, Saharanpur, 247 001, India
Gautam, A., Department of Paper Technology, Indian Institute of Technology Roorkee, Saharanpur Campus, Saharanpur, 247 001, India
Funding Details: The authors are thankful to Jawaharlal Nehru Memorial Fund , Teen Murti House, New Delhi for awarding scholarship to carry out the present work.
Corresponding Author: Kumar, A.; Department of Paper Technology, Indian Institute of Technology Roorkee, Saharanpur Campus, India; email: baliyaniitr@gmail.com
Appears in Collections:Journal Publications [PT]

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