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Please use this identifier to cite or link to this item: http://repository.iitr.ac.in/handle/123456789/13321
Title: Production and characterization of cellulase-free xylanase by aspergillus flavus ARC-12 and its application in pre-bleaching of ethanol-soda pulp of eulaliopsis binata
Authors: Gautam A.
Kumar A.
Dutt D.
Published in: Research Journal of Biotechnology
Abstract: Pre-Treatment of pulp with cellulase-free xylanases can minimize the consumption of bleaching chemicals. A. flavus ARC-12 produced xylanase (1699.50 IU/gds) using pearl millet stover as the carbon source under SSF while cellulase activity was not detected in the crude xylanase. Xylanase production was enhanced up to 2219.85 IU/gds by the addition of beef extract (1.2%, w/v) as nitrogen sources. Xylanase production by A. flavus ARC-12 was further improved up to 2539.54 IU/gds by supplementing 0.10% (w/v) Tween-60 as surfactant. Partially purified xylanase showed optimum activity at pH 6.0 and temperature 50 °C. Xylanase was stable at 50 °C for a longer time (180 min) while declined activity drastically at 55 and 60°C at incubation time of 60 min. Crude xylanase was utilized effectively in the pre-bleaching of ethanol-soda pulp of E. binata. At an enzyme dose of 10 IU/g, kappa number was decreased by 18.51% while pulp brightness and viscosity were improved by 4.2% (ISO) and 4.96% respectively.
Citation: Research Journal of Biotechnology (2017), 12(8): 63-71
URI: http://repository.iitr.ac.in/handle/123456789/13321
Issue Date: 2017
Publisher: Research Journal of BioTechnology
Keywords: Aspergillus flavus
Eulaliopsis binata
Pre-bleaching
Surfactants
Xylanase
ISSN: 9736263
Author Scopus IDs: 57198173170
57206266862
7005982560
Author Affiliations: Gautam, A., Department of Paper Technology, Indian Institute of Technology Roorkee, Saharanpur Campus, Saharanpur, 247001, India
Kumar, A., Department of Paper Technology, Indian Institute of Technology Roorkee, Saharanpur Campus, Saharanpur, 247001, India, Department of Biotechnology, Maharishi Markandeshwar University, Mullana-Ambala, 133207, India
Dutt, D., Department of Paper Technology, Indian Institute of Technology Roorkee, Saharanpur Campus, Saharanpur, 247001, India
Appears in Collections:Journal Publications [PT]

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