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Please use this identifier to cite or link to this item: http://repository.iitr.ac.in/handle/123456789/13320
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dc.contributor.authorAgnihotri S.-
dc.contributor.authorDutt, Dharm-
dc.contributor.authorTyagi C.H.-
dc.contributor.authorKumar A.-
dc.contributor.authorUpadhyaya J.S.-
dc.date.accessioned2020-10-15T12:31:35Z-
dc.date.available2020-10-15T12:31:35Z-
dc.date.issued2010-
dc.identifier.citationWorld Journal of Microbiology and Biotechnology (2010), 26(8): 1349-1359-
dc.identifier.issn9593993-
dc.identifier.urihttps://doi.org/10.1007/s11274-010-0307-9-
dc.identifier.urihttp://repository.iitr.ac.in/handle/123456789/13320-
dc.description.abstractFungi producing xylanases are plentiful but alkali-thermo-tolerant fungi producing cellulase-poor xylanase are rare. Out of 12 fungal strains isolated from various sources, Coprinellus disseminatus SW-1 NTCC 1165 yielded the highest xylanase activity (362.1 IU/ml) with minimal cellulase contamination (0.64 IU/ml). The solid state fermentation was more effective yielding 88.59% higher xylanase activity than that of submerged fermentation. An incubation period of 7 days at 37°C and pH 6.4 accelerated the xylanase production up to the maximum level. Among various inexpensive agro-residues used as carbon source, wheat bran induced the maximum xylanase titres (469.45 IU/ml) while soya bean meal was the best nitrogen source (478.5 IU/ml). A solid substrate to moisture content ratio of 1:3 was suitable for xylanase production while xylanase titre was repressed with the addition of glucose and lactose. The xylanase and laccase activities under optimized conditions were 499.60 and 25.5 IU/ml, respectively along with negligible cellulase contamination (0.86 IU/ml). Biochemical characterization revealed that optimal xylanase activity was observed at pH 6.4 and temperature 55°C and xylanase is active up to pH 9 (40.33 IU/ml) and temperature 85°C (48.81 IU/ml). SDS-PAGE and zymogram analysis indicated that molecular weight of alkali-thermo-tolerant xylanase produced by C. disseminatus SW-1 NTCC 1165 was 43 kDa. © 2010 Springer Science+Business Media B.V.-
dc.language.isoen_US-
dc.relation.ispartofWorld Journal of Microbiology and Biotechnology-
dc.subjectAlkali-thermo-tolerant-
dc.subjectCellulase-
dc.subjectCoprinellus disseminatus-
dc.subjectLaccase-
dc.subjectXylanase-
dc.titleProduction and biochemical characterization of a novel cellulase-poor alkali-thermo-tolerant xylanase from Coprinellus disseminatus SW-1 NTCC 1165-
dc.typeArticle-
dc.scopusid35298446100-
dc.scopusid7005982560-
dc.scopusid6602705260-
dc.scopusid55716707300-
dc.scopusid8679068400-
dc.affiliationAgnihotri, S., Department of Paper Technology, Indian Institute of Technology Roorkee, Saharanpur Campus, Saharanpur 247 001, India-
dc.affiliationDutt, D., Department of Paper Technology, Indian Institute of Technology Roorkee, Saharanpur Campus, Saharanpur 247 001, India-
dc.affiliationTyagi, C.H., Department of Paper Technology, Indian Institute of Technology Roorkee, Saharanpur Campus, Saharanpur 247 001, India-
dc.affiliationKumar, A., Department of Paper Technology, Indian Institute of Technology Roorkee, Saharanpur Campus, Saharanpur 247 001, India-
dc.affiliationUpadhyaya, J.S., Department of Paper Technology, Indian Institute of Technology Roorkee, Saharanpur Campus, Saharanpur 247 001, India-
dc.description.fundingAcknowledgments First author acknowledges Ministry of Human Resource and Development, Government of India for awarding Senior Research Fellowship for conducting this piece of work.-
dc.description.correspondingauthorAgnihotri, S.; Department of Paper Technology, Indian Institute of Technology Roorkee, Saharanpur Campus, Saharanpur 247 001, India; email: swarnimagni@gmail.com-
Appears in Collections:Journal Publications [PT]

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