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Please use this identifier to cite or link to this item: http://repository.iitr.ac.in/handle/123456789/13320
Title: Production and biochemical characterization of a novel cellulase-poor alkali-thermo-tolerant xylanase from Coprinellus disseminatus SW-1 NTCC 1165
Authors: Agnihotri S.
Dutt D.
Tyagi C.H.
Kumar A.
Upadhyaya J.S.
Published in: World Journal of Microbiology and Biotechnology
Abstract: Fungi producing xylanases are plentiful but alkali-thermo-tolerant fungi producing cellulase-poor xylanase are rare. Out of 12 fungal strains isolated from various sources, Coprinellus disseminatus SW-1 NTCC 1165 yielded the highest xylanase activity (362.1 IU/ml) with minimal cellulase contamination (0.64 IU/ml). The solid state fermentation was more effective yielding 88.59% higher xylanase activity than that of submerged fermentation. An incubation period of 7 days at 37°C and pH 6.4 accelerated the xylanase production up to the maximum level. Among various inexpensive agro-residues used as carbon source, wheat bran induced the maximum xylanase titres (469.45 IU/ml) while soya bean meal was the best nitrogen source (478.5 IU/ml). A solid substrate to moisture content ratio of 1:3 was suitable for xylanase production while xylanase titre was repressed with the addition of glucose and lactose. The xylanase and laccase activities under optimized conditions were 499.60 and 25.5 IU/ml, respectively along with negligible cellulase contamination (0.86 IU/ml). Biochemical characterization revealed that optimal xylanase activity was observed at pH 6.4 and temperature 55°C and xylanase is active up to pH 9 (40.33 IU/ml) and temperature 85°C (48.81 IU/ml). SDS-PAGE and zymogram analysis indicated that molecular weight of alkali-thermo-tolerant xylanase produced by C. disseminatus SW-1 NTCC 1165 was 43 kDa. © 2010 Springer Science+Business Media B.V.
Citation: World Journal of Microbiology and Biotechnology (2010), 26(8): 1349-1359
URI: https://doi.org/10.1007/s11274-010-0307-9
http://repository.iitr.ac.in/handle/123456789/13320
Issue Date: 2010
Keywords: Alkali-thermo-tolerant
Cellulase
Coprinellus disseminatus
Laccase
Xylanase
ISSN: 9593993
Author Scopus IDs: 35298446100
7005982560
6602705260
55716707300
8679068400
Author Affiliations: Agnihotri, S., Department of Paper Technology, Indian Institute of Technology Roorkee, Saharanpur Campus, Saharanpur 247 001, India
Dutt, D., Department of Paper Technology, Indian Institute of Technology Roorkee, Saharanpur Campus, Saharanpur 247 001, India
Tyagi, C.H., Department of Paper Technology, Indian Institute of Technology Roorkee, Saharanpur Campus, Saharanpur 247 001, India
Kumar, A., Department of Paper Technology, Indian Institute of Technology Roorkee, Saharanpur Campus, Saharanpur 247 001, India
Upadhyaya, J.S., Department of Paper Technology, Indian Institute of Technology Roorkee, Saharanpur Campus, Saharanpur 247 001, India
Funding Details: Acknowledgments First author acknowledges Ministry of Human Resource and Development, Government of India for awarding Senior Research Fellowship for conducting this piece of work.
Corresponding Author: Agnihotri, S.; Department of Paper Technology, Indian Institute of Technology Roorkee, Saharanpur Campus, Saharanpur 247 001, India; email: swarnimagni@gmail.com
Appears in Collections:Journal Publications [PT]

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