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Please use this identifier to cite or link to this item: http://repository.iitr.ac.in/handle/123456789/13281
Title: Isolation and biochemical characterization of crude xylanase from Coprinus cinereus AT-1 MTCC 9695 and its effectiveness in biodeinking of SOP
Authors: Dutt, Dharm
Tyagi C.H.
Singh R.P.
Gautam A.
Agnohotri S.
Kumar A.
Published in: Cellulose Chemistry and Technology
Abstract: Out of 10 fungal strains isolated from various sources, Coprinus cinereus AT-1 MTCC 9695 produced the highest xylanase (698.75 IU/mL), cellulase (1.01 IU/mL) and laccase (25.6 ±3.2 IU/mL) activities, using wheat bran and yeast extract as the carbon and nitrogen sources at incubation period of 7days, incubation temperature of 37 °C, pH 6.4 and solid substrate to NSS ratio of 1:3. The SSF was more effective, yielding 54.55% higher xylanase activity, compared to SmF. Biochemical characterization revealed that crude xylanase was active in the pH range of 6.0 to 8.0 with the maximum xylanase activity at pH 6.4 (699.60 IU/mL). At neutral and 7.5 pH levels, xylanase retained about 64 and 56% xylanase activity, respectively. At a temperature of 65 °C, xylanase retained about 74% of its optimum xylanase activity and at higher temperatures (75 and 85 °C), it retained 17 and 11% of its optimum activity, respectively. SDS-PAGE and zymogram analysis indicated that the molecular weights of xylanase produced by Coprinus cinereus AT-1 MTCC 9695 were of 50 kDa and 83 kDa. The effectiveness of crude xylanase was assessed by adding it along with cellulase in equal amounts (6 IU/mL) during deinking of SOP. It mitigated ERIC values and dirt counts by 11.34 and 2.20% and improved brightness, DB and DE by 3.07, 8.73 and 4.90%, respectively, compared to deinking with cellulase only.
Citation: Cellulose Chemistry and Technology (2013), 47(43894): 203-217
URI: http://repository.iitr.ac.in/handle/123456789/13281
Issue Date: 2013
Keywords: Brightness
Cellulase
Coprinus cinereus
Deinking
Dirt counts
Xylanase
ISSN: 5769787
Author Scopus IDs: 7005982560
6602705260
57211196378
57198173170
55799346600
57206266862
Author Affiliations: Dutt, D., Department of Paper Technology, Indian Institute of Technology Roorkee, Saharanpur Casmpus, Saharanpur 247001, India
Tyagi, C.H., Department of Paper Technology, Indian Institute of Technology Roorkee, Saharanpur Casmpus, Saharanpur 247001, India
Singh, R.P., Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, India
Gautam, A., Department of Paper Technology, Indian Institute of Technology Roorkee, Saharanpur Casmpus, Saharanpur 247001, India
Agnohotri, S., NTNU, Department of Chemical Engineering, Sem Saelands vei 4, No-7491, Trondheim, Norway
Kumar, A., Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, India
Corresponding Author: Department of Paper Technology, Indian Institute of Technology Roorkee, Saharanpur Casmpus, Saharanpur 247001, India
Appears in Collections:Journal Publications [PT]

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