http://repository.iitr.ac.in/handle/123456789/1301| Title: | Differential neural cell adhesion and neurite outgrowth on carbon nanotube and graphene reinforced polymeric scaffolds |
| Authors: | Gupta P. Agrawal A. Murali K. Varshney R. Beniwal S. Manhas, Sanjeev Kumar Roy, Partha Lahiri, Debrupa |
| Published in: | Materials Science and Engineering C |
| Abstract: | Carbon nanomaterials, such as graphene nanoplatelets (GNPs) and multiwalled carbon nanotubes (MWCNTs) are potential candidates in a large number of biomedical applications. The present study investigates the effect of the difference in morphology of these materials on neural cell regeneration on a biodegradable scaffold. Electrical conductivities of all the hybrid scaffolds are found to be in between that of MWCNT/chitosan scaffold (highest-conductivity) and GNP/chitosan scaffold (lowest-conductivity). While, hybrid scaffolds show improvement in elastic modulus and ultimate tensile strength over MWCNT/chitosan and GNP/chitosan scaffolds. The protein adsorption isotherms of bovine serum albumin (BSA) show greater equilibrium constant (Keq) on GNP/chitosan composites as compared to MWCNT/chitosan composites, proving more potential for cell adhesion in the former. Interactions of HT-22 hippocampal neurons with MWCNT/chitosan, GNP/chitosan and various MWCNT/GNP hybrid chitosan matrices prove cytocompatibility. The neurons acquire elongated geometry on the MWCNT/chitosan scaffold, while GNP reinforcement drives the neurons to spread cellular processes radially. © 2018 Elsevier B.V. |
| Citation: | Materials Science and Engineering C (2019), 97(): 539-551 |
| URI: | https://doi.org/10.1016/j.msec.2018.12.065 http://repository.iitr.ac.in/handle/123456789/1301 |
| Issue Date: | 2019 |
| Publisher: | Elsevier Ltd |
| Keywords: | Electrical conductivity Graphene nanoplatelet Mechanical property Multiwalled carbon nanotube Neural scaffold Shape dependency |
| ISSN: | 9284931 |
| Author Scopus IDs: | 57206494195 57205205543 8872598700 57191823442 57195127101 6602269066 35509207200 26326345700 |
| Author Affiliations: | Gupta, P., Centre of Nanotechnology, IIT Roorkee, India, Biomaterials and Multiscale Mechanics Lab, Department of Metallurgical and Materials Engineering, IIT Roorkee, India Agrawal, A., University of Petroleum and Energy Studies, Dehradun, India Murali, K., Centre of Nanotechnology, IIT Roorkee, India, Biomaterials and Multiscale Mechanics Lab, Department of Metallurgical and Materials Engineering, IIT Roorkee, India, Department of Biotechnology, IIT Roorkee, India Varshney, R., Department of Biotechnology, IIT Roorkee, India Beniwal, S., Department of Electronics and Communication Engineering, IIT Roorkee, Roorkee, Uttarakhand 247667, India Manhas, S., Department of Electronics and Communication Engineering, IIT Roorkee, Roorkee, Uttarakhand 247667, India Roy, P., Centre of Nanotechnology, IIT Roorkee, India, Department of Biotechnology, IIT Roorkee, India Lahiri, D., Centre of Nanotechnology, IIT Roorkee, India, Biomaterials and Multiscale Mechanics Lab, Department of Metallurgical and Materials Engineering, IIT Roorkee, India |
| Funding Details: | Pallavi Gupta acknowledges Dr. Arvind Agarwal, Professor, Florida International University, USA, and Cnano Technology Limited, Beijing, People's Republic of China, for generously providing CNTs for this research. The author also acknowledges the financial support from the Indian Council of Medical Research ( ICM-1033-NTC ) and Faculty Initiation Grant ( FIG-100613 ) by IIT Roorkee for carrying out this research. The authors acknowledge Dr. Dave Schubert, The Salk Institute, La Jolla, CA, USA, for providing HT-22 cell line through Dr. S. K. Sharma, National Brain Research Center, Gurgaon, India, used in this research. The authors also wish to thank the laboratory staff of the Centre for Nanotechnology, Department of Metallurgical and Materials Engineering and Department of Electronics and Communication Engineering, IIT Roorkee, for maintaining the experimental facilities. |
| Corresponding Author: | Gupta, P.; Centre of Nanotechnology, IIT RoorkeeIndia; email: pgupta1304@gmail.com |
| Appears in Collections: | Journal Publications [BT] Journal Publications [ECE] Journal Publications [MT] |
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