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Title: Valorization of food-waste hydrolysate by Lentibacillus salarius NS12IITR for the production of branched chain fatty acid enriched lipid with potential application as a feedstock for improved biodiesel
Authors: Singh N.
Choudhury, Bijan
Published in: Waste Management
Abstract: Oxidation stability and cold flow properties of biodiesel can be improved by using lipid with enriched branched-chain fatty acid (BCFA) as a feedstock. A halophilic bacterium was utilized for the production of BCFA enriched lipid from acid hydrolysate of food-waste. The maximum reducing sugar obtained by hydrolysis of wheat bran, rice bran, mango peel, and orange peel were 64.52 ± 0.57, 38.7 ± 0.58, 55.64 ± 1.14, 36.29 ± 0.54 g/L, respectively. On assessing these hydrolysates as feedstock for growth of halophilic bacterium Lentibacillus salarius NS12IITR at 10 g/L reducing sugar concentration, wheat bran hydrolysate was found to be best in-terms of sugar consumption (92%), lipid production (0.70 ± 0.029 g/L) and maximum branched-chain fatty acid methyl ester (FAME) (81 ± 4.72% of total FAME). At 20 g/L of reducing sugar concentration of wheat bran hydrolysate, the biomass and lipid yields were almost doubled. Efficient lipid extraction from cell, involving thermolysis at 85 °C and pH 2 along with osmotic shock resulted in isolation of 69% of total lipid. © 2019 Elsevier Ltd
Citation: Waste Management (2019), 94(): 1-9
Issue Date: 2019
Publisher: Elsevier Ltd
Keywords: Cell disruption
Dilute acid hydrolysis
Food-waste hydrolysate
Lentibacillus salarius
ISSN: 0956053X
Author Scopus IDs: 57213672802
Author Affiliations: Singh, N., Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand 247667, India
Choudhury, B., Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand 247667, India
Funding Details: The authors gratefully acknowledge the financial support from IIT Roorkee , India and Govt. of India , India for providing financial support.
Corresponding Author: Choudhury, B.; Department of Biotechnology, Indian Institute of Technology RoorkeeIndia; email:
Appears in Collections:Journal Publications [BT]

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