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Please use this identifier to cite or link to this item: http://repository.iitr.ac.in/handle/123456789/12608
Title: Aligned carbon nanotube reinforced polymeric scaffolds with electrical cues for neural tissue regeneration
Authors: Gupta P.
Sharan S.
Roy, Partha
Lahiri, Debrupa
Published in: Carbon
Abstract: In this article, the challenge of providing electrical cue through a soft polymeric neural scaffold for directional neuron growth has been studied. Aligned multiwalled carbon nanotube (MWCNT) chitosan composite scaffolds are successfully fabricated using electric field alignment technique. Uniform distribution and alignment of 0.5 wt% MWCNTs in the chitosan matrix, good interfacial bonding, and aligned network of carbon nanotubes (CNTs) help to improve the elastic modulus, yield strength, and ultimate tensile strength by 12.7%, 21.9%, and 11.2%, respectively, as compared with the random MWCNT-chitosan scaffold. Alignment of MWCNTs introduces highly anisotropic electrical conductivity (100,000 times higher) along its direction, as compared to the transverse direction of the scaffold. This is the ideal requirement for a neural scaffold to guide cells in the appropriate direction. Interactions of HT-22 hippocampal neurons with MWCNT-chitosan matrix prove the scaffolds to be highly biocompatible with a notable increase in viability. In addition, 50-60% of neurons are found to be aligned in the MWCNT alignment direction of the scaffold. These results indicate that aligned MWCNT-chitosan scaffold is a potential design to control the directional cell migration on the scaffold, which opens up the possibility of its use in repopulating cells in regions of acute neuronal loss. © 2015 Elsevier Ltd. All rights reserved.
Citation: Carbon (2015), 95(): 715-724
URI: https://doi.org/10.1016/j.carbon.2015.08.107
http://repository.iitr.ac.in/handle/123456789/12608
Issue Date: 2015
Publisher: Elsevier Ltd
ISSN: 86223
Author Scopus IDs: 57206494195
55661549600
35509207200
26326345700
Author Affiliations: Gupta, P., Centre of Nanotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand, 247667, India, Biomaterials and Multiscale Mechanics Lab, Department of Metallurgical and Materials Engineering, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand, 247667, India
Sharan, S., Molecular Endocrinology Lab, Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand, 247667, India
Roy, P., Centre of Nanotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand, 247667, India, Molecular Endocrinology Lab, Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand, 247667, India
Lahiri, D., Centre of Nanotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand, 247667, India, Biomaterials and Multiscale Mechanics Lab, Department of Metallurgical and Materials Engineering, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand, 247667, India
Funding Details: Debrupa Lahiri acknowledges Dr Arvind Agarwal, Professor, Florida International University, USA, and Cnano Technology Limited, Beijing, People's Republic of China, for generously providing CNTs for this research. The author also acknowledges the financial support from the Faculty Initiation Grant ( FIG-100613 ) by IIT Roorkee for carrying out this research. The authors acknowledge Dr Dave Schubert, The Salk Institute, La Jolla, CA, USA, for providing HT-22 cell line through Dr S. K. Sharma, National Brain Research Center, Gurgaon, India, used in this research. The authors appreciate the support from Mr Vijayesh Kumar, PhD Scholar, Centre for Nanotechnology, IIT Roorkee, Mr Snehashish Das, PhD Scholar, Department of Biotechnology, IIT Roorkee, for their help during initial studies. The authors also wish to thank the laboratory staff of the Centre for Nanotechnology and the Department of Metallurgical and Materials Engineering, IIT Roorkee, for maintaining the experimental facilities.
Corresponding Author: Lahiri, D.; Centre of Nanotechnology, Indian Institute of Technology RoorkeeIndia
Appears in Collections:Journal Publications [BT]
Journal Publications [MT]

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