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Please use this identifier to cite or link to this item: http://repository.iitr.ac.in/handle/123456789/1259
Title: Structural insights into Entamoeba histolytica arginase and structure-based identification of novel non-amino acid based inhibitors as potential antiamoebic molecules
Authors: Malik A.
Dalal V.
Ankri S.
Tomar, Shailly
Published in: FEBS Journal
Abstract: Arginase, the binuclear metalloenzyme, is a potential target for therapeutic intervention in protozoan infections. Entamoeba histolytica infection causes amebiasis which is the second most common cause of protozoan-related human deaths after malaria. Here, we report the crystal structure of E. histolytica arginase (EhArg) in complex with two known inhibitors N?-hydroxy-l-arginine (l-NOHA) and l-norvaline, and its product l-ornithine at 1.7, 2.0, and 2.4 Å, respectively. Structural and comparative analysis of EhArg–inhibitor complexes with human arginase revealed that despite only 33% sequence identity, the structural determinants of inhibitor recognition and binding are highly conserved in arginases with variation in oligomerization motifs. Knowledge regarding the spatial organization of residues making molecular contacts with inhibitory compounds enabled in the identification of four novel non-amino acid inhibitors, namely irinotecan, argatroban, cortisone acetate, and sorafenib. In vitro testing of the in silico-identified inhibitors using purified enzyme proved that irinotecan, argatroban, cortisone acetate, and sorafenib inhibit EhArg with IC50 value (mm) of 1.99, 2.40, 0.91, and 2.75, respectively, as compared to the known inhibitors l-NOHA and l-norvaline with IC50 value (mm) of 1.57 and 17.9, respectively. The identification of structure-based non-amino acid inhibitory molecules against arginase will be constructive in design and discovery of novel chemical modulators for treating amebiasis by directed therapeutics. © 2019 Federation of European Biochemical Societies
Citation: FEBS Journal(2019), 286(20): 4135-4155
URI: https://doi.org/10.1111/febs.14960
http://repository.iitr.ac.in/handle/123456789/1259
Issue Date: 2019
Publisher: Blackwell Publishing Ltd
Keywords: arginase
crystal structure
Entamoeba histolytica
l-norvaline
structure-based drug designing
ISSN: 1742464X
Author Scopus IDs: 57191902133
57194227044
6701672273
57203506001
Author Affiliations: Malik, A., Department of Biotechnology, Indian Institute of Technology Roorkee, India
Dalal, V., Department of Biotechnology, Indian Institute of Technology Roorkee, India
Ankri, S., Department of Molecular Microbiology, Bruce Rappaport Faculty of Med
Funding Details: AM thanks University Grant Commission (UGC) and VD thanks the Department of Biotechnology (DBT/2015/IIT-R/349) for financial support. AM and VD thank Macromolecular Crystallographic Unit (MCU), a Central Facility at Institute Instrumentation Centre (IIC),
Corresponding Author: Tomar, S.; Department of Biotechnology, Indian Institute of Technology RoorkeeIndia; email: shailfbt@iitr.ac.in
Appears in Collections:Journal Publications [BT]

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