Skip navigation
Please use this identifier to cite or link to this item: http://repository.iitr.ac.in/handle/123456789/1254
Title: A combination of linalool, vitamin C, and copper synergistically triggers reactive oxygen species and DNA damage and inhibits Salmonella enterica subsp. enterica serovar typhi and Vibrio fluvialis
Authors: Ghosh T.
Srivastava S.K.
Gaurav A.
Kumar A.
Kumar P.
Yadav A.S.
Pathania, R.
Navani, Naveen Kumar
Published in: Applied and Environmental Microbiology
Abstract: Inappropriate and disproportionate use of antibiotics is contributing immensely to the development of antibiotic resistance in bacterial species associated with food contamination. The use of natural products in combination can be a potent alternative hurdle strategy to inactivate foodborne pathogens. Here, we explored the pro-oxidant properties of essential oil linalool and vitamin C in combination with copper (LVC) in combating the foodborne pathogens Vibrio fluvialis and Salmonella enterica subsp. enterica serovar Typhi using a three-dimensional (3D) checkerboard microdilution assay. Antibacterial activity in terms of the MIC revealed that the triple combination exerted a synergistic effect compared to the effects of the individual constituents. The bactericidal effect of the triple combination was confirmed by a live/dead staining assay. Reactive oxygen species (ROS) measurements with the terminal deoxynucleotidyltransferase-mediated dUTP-biotin nick end labeling assay and scanning electron microscopy imaging strongly suggested that the increase in ROS production is the underlying mechanism of the enhanced antibacterial potency of the LVC combination (linalool [1.298 mM], vitamin C [8 mM], copper [16.3 ?M]). In addition, the hypersensitivity of oxidative stress regulator mutants (oxyR, katG, ahpC, and sodA mutants) toward LVC corroborated the involvement of ROS in cell death. Live/dead staining and changes in cellular morphology revealed that oxidative stress did not transform the cells into the viable but nonculturable (VBNC) state; rather, killing was associated with intracellular and extracellular oxidative burst. Furthermore, the LVC combination did not display toxicity to human cells, while it effectively reduced the pathogen levels in acidic fruit juices by 3 to 4 log CFU/ml without adversely altering the organoleptic properties. This study opens a new outlook for combinatorial antimicrobial therapy. © 2019 American Society for Microbiology. All Rights Reserved.
Citation: Applied and Environmental Microbiology (2019), 85(4): -
URI: https://doi.org/10.1128/AEM.02487-18
http://repository.iitr.ac.in/handle/123456789/1254
Issue Date: 2019
Publisher: American Society for Microbiology
Keywords: Antimicrobial agents
Copper
Essential oil
Foodborne pathogens
Oxidative stress
ROS
Salmonella enterica subsp. enterica serovar Typhi
Vibrio fluvialis
Vitamin C
ISSN: 992240
Author Scopus IDs: 57197638847
57202143131
57205694539
55716707300
57216399468
35202571100
7004308029
6507073924
Author Affiliations: Ghosh, T., Chemical Biology Laboratory, Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand, India
Srivastava, S.K., Chemical Biology Laboratory, Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand, India
Gaurav, A., Molecular Bacteriology and oChemical Genetics Laboratory, Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand, India
Kumar, A., Chemical Biology Laboratory, Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand, India
Kumar, P., Chemical Biology Laboratory, Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand, India
Yadav, A.S., Post-Harvest Technology Division, ICAR-Central Avian Research Institute, Izatnagar, Bareilly, Uttar Pradesh, India
Pathania, R., Molecular Bacteriology and oChemical Genetics Laboratory, Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand, India
Navani, N.K., Chemical Biology Laboratory, Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand, India
Corresponding Author: Navani, N.K.; Chemical Biology Laboratory, Department of Biotechnology, Indian Institute of Technology RoorkeeIndia; email: navnifbs@iitr.ac.in
Appears in Collections:Journal Publications [BT]

Files in This Item:
There are no files associated with this item.
Show full item record


Items in Repository are protected by copyright, with all rights reserved, unless otherwise indicated.