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Please use this identifier to cite or link to this item: http://repository.iitr.ac.in/handle/123456789/1236
Title: Delineating the molecular responses of a halotolerant microalga using integrated omics approach to identify genetic engineering targets for enhanced TAG production 06 Biological Sciences 0601 Biochemistry and Cell Biology 06 Biological Sciences 0607 Plant
Authors: Arora N.
Kumari P.
Kumar A.
Gangwar R.
Gulati K.
Pruthi P.A.
Prasad, Ramasare A.
Kumar D.
Pruthi V.
Poluri, Krishna Mohan
Published in: Biotechnology for Biofuels
Abstract: Background: Harnessing the halotolerant characteristics of microalgae provides a viable alternative for sustainable biomass and triacylglyceride (TAG) production. Scenedesmus sp. IITRIND2 is a fast growing fresh water microalga that has the capability to thrive in high saline environments. To understand the microalga's adaptability, we studied its physiological and metabolic flexibility by studying differential protein, metabolite and lipid expression profiles using metabolomics, proteomics, real-time polymerase chain reaction, and lipidomics under high salinity conditions. Results: On exposure to salinity, the microalga rewired its cellular reserves and ultrastructure, restricted the ions channels, and modulated its surface potential along with secretion of extrapolysaccharide to maintain homeostasis and resolve the cellular damage. The algal-omics studies suggested a well-organized salinity-driven metabolic adjustment by the microalga starting from increasing the negatively charged lipids, up regulation of proline and sugars accumulation, followed by direction of carbon and energy flux towards TAG synthesis. Furthermore, the omics studies indicated both de-novo and lipid cycling pathways at work for increasing the overall TAG accumulation inside the microalgal cells. Conclusion: The salt response observed here is unique and is different from the well-known halotolerant microalga; Dunaliella salina, implying diversity in algal response with species. Based on the integrated algal-omics studies, four potential genetic targets belonging to two different metabolic pathways (salt tolerance and lipid production) were identified, which can be further tested in non-halotolerant algal strains. © 2019 The Author(s).
Citation: Biotechnology for Biofuels(2019), 12(1): -
URI: https://doi.org/10.1186/s13068-018-1343-1
http://repository.iitr.ac.in/handle/123456789/1236
Issue Date: 2019
Publisher: BioMed Central Ltd.
Keywords: Algal-omics
Genetic engineering
Halotolerant
Microalga
Scenedesmus sp. IITRIND2
Sea water
TAG production
ISSN: 17546834
Author Scopus IDs: 56800005300
57210238723
57202315717
57196954226
56998446900
25958285300
16690394400
17035060600
18837702800
55842079400
Author Affiliations: Arora, N., Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand, 247667, India
Kumari, P., Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand, 247667, India
Kumar, A., Centre
Funding Details: We acknowledge the support of FE-SEM, ICP-MS, and NMR instrumentation facilities at Institute instrumentation centre (IIC), IIT-Roorkee. We are highly thankful for the access to 800 MHz NMR at CMBR, Lucknow, and TEM facility at AIIMS, New Delhi.
Corresponding Author: Kumar, D.; Centre of Biomedical Research, SGPGIMSIndia; email: dineshcbmr@gmail.com
Appears in Collections:Journal Publications [BT]

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