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Please use this identifier to cite or link to this item: http://repository.iitr.ac.in/handle/123456789/1150
Title: Identification of novel regulatory small RNAs in Acinetobacter baumannii
Authors: Sharma R.
Arya S.
Patil S.D.
Sharma A.
Jain P.K.
Navani N.K.
Pathania R.
Published in: PLoS ONE
Abstract: Small RNA (sRNA) molecules are non-coding RNAs that have been implicated in regulation of various cellular processes in living systems, allowing them to adapt to changing environmental conditions. Till date, sRNAs have not been reported in Acinetobacter baumannii ( A. baumannii ), which has emerged as a significant multiple drug resistant nosocomial pathogen. In the present study, a combination of bioinformatic and experimental approach was used for identification of novel sRNAs. A total of 31 putative sRNAs were predicted by a combination of two algorithms, sRNAPredict and QRNA. Initially 10 sRNAs were chosen on the basis of lower E- value and three sRNAs (designated as AbsR11, 25 and 28) showed positive signal on Northern blot. These sRNAs are novel in nature as they do not have homologous sequences in other bacterial species. Expression of the three sRNAs was examined in various phases of bacterial growth. Further, the effect of various stress conditions on sRNA gene expression was determined. A detailed investigation revealed differential expression profile of AbsR25 in presence of varying amounts of ethidium bromide (EtBr), suggesting that its expression is influenced by environmental or internal signals such as stress response. A decrease in expression of AbsR25 and concomitant increase in the expression of bioinformatically predicted targets in presence of high EtBr was reverberated by the decrease in target gene expression when AbsR25 was overexpressed. This hints at the negative regulation of target genes by AbsR25. Interestingly, the putative targets include transporter genes and the degree of variation in expression of one of them (A1S-1331) suggests that AbsR25 is involved in regulation of a transporter. This study provides a perspective for future studies of sRNAs and their possible involvement in regulation of antibiotic resistance in bacteria specifically in cryptic A. baumannii. © 2014 Sharma et al.
Citation: PLoS ONE (2014), 9(4): -
URI: https://doi.org/10.1371/journal.pone.0093833
http://repository.iitr.ac.in/handle/123456789/1150
Issue Date: 2014
Publisher: Public Library of Science
ISSN: 19326203
Author Scopus IDs: 57212897407
56126612900
55614242900
57217481823
57199743190
6507073924
7004308029
Author Affiliations: Sharma, R., Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand, India
Arya, S., Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand, India
Patil, S.D., Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand, India
Sharma, A., Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand, India
Jain, P.K., National Research Centre on Plant Biotechnology, New Delhi, India
Navani, N.K., Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand, India
Pathania, R., Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand, India
Appears in Collections:Journal Publications [BT]

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