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Please use this identifier to cite or link to this item: http://repository.iitr.ac.in/handle/123456789/11112
Title: Combining Ascochyta blight and Botrytis grey mould resistance in chickpea through interspecific hybridization
Authors: Kaur L.
Sirari A.
Kumar D.
Singh Sandhu J.
Singh S.
Kapoor K.
Singh I.
Gowda C.L.L.
Pande S.
Gaur P.
Sharma M.
Imtiaz M.
Siddique K.H.M.
Published in: Phytopathologia Mediterranea
Abstract: Ascochyta blight (AB) caused by Ascochyta rabiei (Pass.) Labr. and Botrytis grey mould (BGM) caused by Botrytis cinerea (Pers. ex Fr.) are important diseases of the aerial plant parts of chickpea in most chickpea growing areas of the world. Although conventional approaches have contributed to reducing disease, the use of new technologies is expected to further reduce losses through these biotic stresses. Reliable screening techniques were developed: 'field screening technique' for adult plant screening, 'cloth chamber technique' and 'growth chamber technique' for the study of races of the pathogen and for segregating generations. Furthermore, the 'cut twig technique' for interspecific population for AB and BGM resistance was developed. For introgression of high levels of AB and BGM resistance in cultivated chickpea from wild relatives, accessions of seven annual wild Cicer spp. were evaluated and identified: C. judaicum accessions 185, ILWC 95 and ILWC 61, C. pinnatifidum accessions 188, 199 and ILWC 212 as potential donors. C. pinnatifidum accession188 was crossed with ICCV 96030 and 62 F9 lines resistant to AB and BGM were derived. Of the derived lines, several are being evaluated for agronomic traits and yield parameters while four lines, GL 29029, GL29206, GL29212, GL29081 possessing high degree of resistance were crossed with susceptible high yielding cultivars BG 256 to improve resistance and to undertake molecular studies. Genotyping of F2 populations with SSR markers from the chickpea genome was done to identify markers potentially linked with AB and BGM resistance genes. In preliminary studies, of 120 SSR markers used, six (Ta 2, Ta 110, Ta 139, CaSTMS 7, CaSTMS 24 and Tr 29) were identified with polymorphic bands between resistant derivative lines and the susceptible parent. The study shows that wild species of Cicer are the valuable gene pools of resistance to AB and BGM. The resistant derivative lines generated here can serve as good pre-breeding material and markers identified can assist in marker assisted selection for resistance breeding. © Firenze University Press.
Citation: Phytopathologia Mediterranea (2013), 52(1): 157-165
URI: http://repository.iitr.ac.in/handle/123456789/11112
Issue Date: 2013
Keywords: Cicer arietinum
Cicer pinnatifidum
Interspecific hybridization
Resistance
SSR markers
ISSN: 319465
Author Scopus IDs: 6602181751
36652101500
57202478211
55744676200
14028945800
55744300400
9943426200
55744980200
55856399600
7003317805
36840989200
24366657000
57203176668
Author Affiliations: Kaur, L., Punjab Agricultural University (PAU), Ludhiana 141004, Punjab, India
Sirari, A., Punjab Agricultural University (PAU), Ludhiana 141004, Punjab, India
Kumar, D., Punjab Agricultural University (PAU), Ludhiana 141004, Punjab, India
Singh Sandhu, J., Indian Council of Agricutural Research, New Delhi 110114, India
Singh, S., Punjab Agricultural University (PAU), Ludhiana 141004, Punjab, India
Kapoor, K., Punjab Agricultural University (PAU), Ludhiana 141004, Punjab, India
Singh, I., Punjab Agricultural University (PAU), Ludhiana 141004, Punjab, India
Gowda, C.L.L., International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru, A.P., India
Pande, S., International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru, A.P., India
Gaur, P., International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru, A.P., India
Sharma, M., International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru, A.P., India
Imtiaz, M., International Centre for Agricultural Research in Dry Areas (ICARDA), Aleppo, Syrian Arab Republic
Siddique, K.H.M., The UWA Institute of Agriculture, The University of Western Australia, 35 Stirling Highway, Crawley, WA 6009, Australia
Corresponding Author: Kaur, L.; Punjab Agricultural University (PAU), Ludhiana 141004, Punjab, India; email: livinderk@rediffmail.com
Appears in Collections:Journal Publications [ME]

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