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Title: Diversity, antimicrobial action and structure- activity relationship of buffalo cathelicidins
Authors: Brahma B.
Patra M.C.
Karri S.
Chopra M.
Mishra P.
De B.C.
Kumar S.
Mahanty S.
Thakur K.
Poluri, Krishna Mohan
Datta T.K.
De S.
Published in: PLoS ONE
Abstract: Cathelicidins are an ancient class of antimicrobial peptides (AMPs) with broad spectrum bactericidal activities. In this study, we investigated the diversity and biological activity of cathelicidins of buffalo, a species known for its disease resistance. A series of new homologs of cathelicidin4 (CATHL4), which were structurally diverse in their antimicrobial domain, was identified in buffalo. AMPs of newly identified buffalo CATHL4s (buCATHL4s) displayed potent antimicrobial activity against selected Gram positive (G+) and Gram negative (G-) bacteria. These peptides were prompt to disrupt the membrane integrity of bacteria and induced specific changes such as blebing, budding, and pore like structure formation on bacterial membrane. The peptides assumed different secondary structure conformations in aqueous and membrane-mimicking environments. Simulation studies suggested that the amphipathic design of buCATHL4 was crucial for water permeation following membrane disruption. A great diversity, broad-spectrum antimicrobial action, and ability to induce an inflammatory response indicated the pleiotropic role of cathelicidins in innate immunity of buffalo. This study suggests short buffalo cathelicidin peptides with potent bactericidal properties and low cytotoxicity have potential translational applications for the development of novel antibiotics and antimicrobial peptidomimetics. © 2015 Brahma et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Citation: PLoS ONE(2015), 10(12): -
Issue Date: 2015
Publisher: Public Library of Science
ISSN: 19326203
Author Scopus IDs: 35114456700
Author Affiliations: Brahma, B., Animal Genomics Lab, Animal Biotechnology Center, National Dairy Research Institute, Karnal, Haryana 132001, India, Krishi Vigyan Kendra, Bhaderwah, Sher-e-Kashmir University of Agricultural Sciences and Technology, Jammu, India
Patra, M.C.
Appears in Collections:Journal Publications [BT]

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