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Please use this identifier to cite or link to this item: http://repository.iitr.ac.in/handle/123456789/10814
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dc.contributor.authorKumar D.-
dc.contributor.authorKumar S.-
dc.contributor.authorChakravarty J.-
dc.contributor.authorSundar S.-
dc.date.accessioned2020-10-15T12:12:19Z-
dc.date.available2020-10-15T12:12:19Z-
dc.date.issued2011-
dc.identifier.citationVector-Borne and Zoonotic Diseases (2011), 11(10): 1359-1364-
dc.identifier.issn15303667-
dc.identifier.other21923256-
dc.identifier.urihttps://doi.org/10.1089/vbz.2011.0620-
dc.identifier.urihttp://repository.iitr.ac.in/handle/123456789/10814-
dc.description.abstractBackground: For the diagnosis of visceral leishmaniasis (VL), rK39 antigen-based rapid test is widely used. Unfortunately, up to 32% healthy individuals from endemic region test positive with this antigen. There is an urgent need to search for a more specific antigen with sensitivity similar to rK39. Methods: We identified a Leishmania donovani-specific 12.6-kDa (BHUP3) soluble promastigote antigen through sensitive western blot technique. The identified protein was partially purified from sodium dodecyl sulfate-polyacrylamide gel electrophoresis and the antigenic response of eluted protein was determined by western blot with different groups of individual sera. The diagnostic potential was further validated by enzyme-linked immunosorbent assay using serum of 100 VL patients, 93 nonendemic healthy control individuals, 110 endemic healthy control individuals, and 110 disease control individuals. Further, it was characterized by two-dimensional gel electrophoresis followed by matrix-assisted laser desorption/ionization-time-of-flight analysis. Results: On blotting, antibody against this protein was recognized by all (9/9) VL patient's sera, but it was absent in every control group (nonendemic healthy control and endemic healthy control). Sensitivity of the enzyme-linked immunosorbent assay was 88% (89/101), whereas the specificity for endemic healthy, nonendemic healthy, and different disease groups were 96% (106/110), 100% (93/93), and 97% (107/110), respectively. The two-dimensional gel electrophoresis showed a single spot, and matrix-assisted laser desorption/ionization-time-of-flight analysis revealed that it is a 113-amino-acid-long putative uncharacterized protein of 12.6-kDa anamorsin homolog matched completely with Leishmania major (GenBank accession number: Q4QIS1). Conclusion: Despite marginally lower sensitivity of BHUP3, excellent specificity warrants its further development as a tool for diagnosis of VL. © Copyright 2011, Mary Ann Liebert, Inc.-
dc.language.isoen_US-
dc.relation.ispartofVector-Borne and Zoonotic Diseases-
dc.subjectDiagnosis-
dc.subjectELISA-
dc.subjectMALDI-TOF-
dc.subjectVisceral leishmaniasis-
dc.subjectWestern blotting-
dc.titleA novel 12.6-kDa protein of leishmania donovani for the diagnosis of Indian visceral leishmaniasis-
dc.typeArticle-
dc.scopusid57202478211-
dc.scopusid57209548091-
dc.scopusid16241108100-
dc.scopusid7103328080-
dc.affiliationKumar, D., Department of Medicine, Institute of Medical Sciences, Banaras Hindu University, Varanasi 221005, Uttar Pradesh, India-
dc.affiliationKumar, S., Department of Medicine, Institute of Medical Sciences, Banaras Hindu University, Varanasi 221005, Uttar Pradesh, India-
dc.affiliationChakravarty, J., Department of Medicine, Institute of Medical Sciences, Banaras Hindu University, Varanasi 221005, Uttar Pradesh, India-
dc.affiliationSundar, S., Department of Medicine, Institute of Medical Sciences, Banaras Hindu University, Varanasi 221005, Uttar Pradesh, India-
dc.description.correspondingauthorSundar, S.; Department of Medicine, Institute of Medical Sciences, Banaras Hindu University, Varanasi 221005, Uttar Pradesh, India; email: drshyamsundar@hotmail.com-
Appears in Collections:Journal Publications [ME]

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