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Please use this identifier to cite or link to this item: http://repository.iitr.ac.in/handle/123456789/1044
Title: Promising ESIPT-based fluorescence sensor for Cu2+ and CN- ions: Investigation towards logic gate behaviour, anticancer activities and bioimaging application
Authors: Bhardwaj S.
Maurya N.
Singh A.K.
Varshney R.
Roy, Partha
Published in: RSC Advances
Abstract: An excited state intramolecular proton transfer (ESIPT) process-based novel chromogenic and fluorogenic probe (2) was synthesized with the aim of sequential in situ detection of Cu2+ and CN- ions under aqueous and biological conditions. The probe revealed chelating affinity for transition metal cations, amidst which only Cu2+ efficiently quenches the emission intensity. Further in situ addition of CN- results in a metal displacement reaction and a turn on fluorescence response. This quencher displacement sensing strategy results in "ON-OFF-ON" type of fluorescence changes with high selectivity and great affinity in nanomolar detection of [CN-] and outlines the working principle of the IMPLICATION logic gate. The in vitro cytotoxic activity studied via the MTT assay revealed that in situ-formed Cu complexes worked as potential anticancer chemotherapeutic agents towards MCF-7 (human breast adenocarcinoma) cell lines (2 + Cu2+ IC50 = 5.69 ± 0.26 ?g mL-1, 3 + Cu2+ IC50 = 7.36 ± 0.29 ?g mL-1). The selective detection of Cu2+ and CN- ions in biological systems was also explained by intracellular bioimaging studies in MCF-7 cell lines. © 2016 The Royal Society of Chemistry.
Citation: RSC Advances (2016), 6(104): 102096-102101
URI: https://doi.org/10.1039/c6ra22352d
http://repository.iitr.ac.in/handle/123456789/1044
Issue Date: 2016
Publisher: Royal Society of Chemistry
ISSN: 20462069
Author Scopus IDs: 56087896600
57117849400
57209223382
57191823442
35509207200
Author Affiliations: Bhardwaj, S., Department of Chemistry, Indian Institute of Technology-Roorkee, Roorkee, 247667, India
Maurya, N., Department of Chemistry, Indian Institute of Technology-Roorkee, Roorkee, 247667, India
Singh, A.K., Department of Chemistry, Indian Institute of Technology-Roorkee, Roorkee, 247667, India
Varshney, R., Department of Biotechnology, Indian Institute of Technology-Roorkee, Roorkee, 247667, India
Roy, P., Department of Biotechnology, Indian Institute of Technology-Roorkee, Roorkee, 247667, India
Corresponding Author: Singh, A.K.; Department of Chemistry, Indian Institute of Technology-RoorkeeIndia; email: akscyfcy@iitr.ernet.in
Appears in Collections:Journal Publications [BT]

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