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Please use this identifier to cite or link to this item: http://repository.iitr.ac.in/handle/123456789/1022
Title: A 500 MHz proton NMR study of stacking interactions: Binding of tripeptide Lys?Tyr?Lys to tetradeoxynucleotide d?GpCpGpC
Authors: Barthwal R.
Mujeeb A.
Kukreti S.
Gupta A.
Govil G.
Published in: Journal of Molecular Recognition
Abstract: The complete sequential assignment and conformation of d?GpCpGpC in D2O has been determined from ID NMR spectra at 285–320 K and room temperature 2D?COSY and NOESY spectra. The tetradeoxynucleotide exists primarily as a right handed double helix at 285 K, having Tm as 314 K. On binding to a tripeptide Lys?Tyr?Lys in a concentration equimolar to tetranucleotide duplex, the Tyr ring protons shift uplifted by 0.14 ppm at 285 K. The increase in Tm on binding suggests stabilization of duplex. The existence of intermolecular NOEs between C4 sugar protons and Tyr ?C and Lys ?C protons give direct evidence of proximity of Tyr residue to the C4 base of d?GpCpGpC. The conformation of d?GpCpGpC remains unchanged on binding. The observed results are interpreted in terms of preferential stacking of aromatic ring of Tyr residue with proximal base?pair of d?GpCpGpC, stabilized by electostatic interaction of Lysine side chains with backbone phosphates. This is in contrast to intercalculation of aromatic dyes within base?pairs resulting in a change in sugar conformation at the binding site. Copyright © 1991 John Wiley & Sons Ltd.
Citation: Journal of Molecular Recognition(1991), 4(43892): 45-52
URI: https://doi.org/10.1002/jmr.300040202
http://repository.iitr.ac.in/handle/123456789/1022
Issue Date: 1991
ISSN: 9523499
Author Scopus IDs: 6602599742
6602736133
6701496717
56183857300
7003994734
Author Affiliations: Barthwal, R., Department of Bioscience and Biotechnology, University of Roorkee, Roorkee, 247667, India
Mujeeb, A., Department of Bioscience and Biotechnology, University of Roorkee, Roorkee, 247667, India
Kukreti, S., Department of Bioscience and Bio
Corresponding Author: Barthwal, R.; Department of Bioscience and Biotechnology, University of Roorkee, Roorkee, 247667, India
Appears in Collections:Journal Publications [BT]

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