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Please use this identifier to cite or link to this item: http://repository.iitr.ac.in/handle/123456789/1018
Title: Purification, crystallization and preliminary X-ray diffraction studies of disintegrin (schistatin) from saw-scaled viper (Echis carinatus)
Authors: Tomar S.
Yadav S.
Chandra V.
Kumar P.
Singh T.P.
Published in: Acta Crystallographica Section D: Biological Crystallography
Abstract: This is the first report of crystallographic data on a disintegrin molecule from any source. The heterodimeric disintegrin with a molecular weight of 14 kDa from Echis carinatus venom is a potent antagonist of ?4 integrins. The intact disintegrin, containing two subunits A and B, was isolated and purified using affinity and ion-exchange columns. It has been crystallized using 1.6 M ammonium sulfate as a precipitating agent. The crystals grew to dimensions of 0.25 × 0.20 × 0.20 mm and diffracted to 2.5 Å resolution. The crystals belong to space group I4122, with unit-cell parameters a = b = 91.7, c = 55.1 Å. Assuming a molecular weight of 14 kDa, a VM of 2.1 Å3 Da-1 is obtained for one molecule of disintegrin in the asymmetric unit.
Citation: Acta Crystallographica Section D: Biological Crystallography(2001), 57(11): 1669-1670
URI: https://doi.org/10.1107/S0907444901012082
http://repository.iitr.ac.in/handle/123456789/1018
Issue Date: 2001
ISSN: 9074449
Author Scopus IDs: 57203506001
37162551900
57197361769
55064809000
35462820700
Author Affiliations: Tomar, S., Department of Biophysics, All India Inst. of Medical Sciences, New Delhi 110 029, India
Yadav, S., Department of Biophysics, All India Inst. of Medical Sciences, New Delhi 110 029, India
Chandra, V., Department of Biophysics, All India Inst
Corresponding Author: Singh, T.P.; Department of Biophysics, All India Inst. of Medical Sciences, New Delhi 110 029, India; email: tps@aiims.aiims.ac.in
Appears in Collections:Journal Publications [BT]

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