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Please use this identifier to cite or link to this item: http://repository.iitr.ac.in/handle/123456789/1010
Title: A novel function for globulin in sequestering plant hormone: Crystal structure of Wrightia tinctoria 11S globulin in complex with auxin
Authors: Kumar P.
Kesari P.
Dhindwal S.
Choudhary A.K.
Katiki M.
Neetu
Verma A.
Ambatipudi K.
Tomar S.
Sharma A.K.
Mishra G.
Kumar P.
Published in: Scientific Reports
Abstract: Auxin levels are tightly regulated within the plant cell, and its storage in the isolated cavity of proteins is a measure adopted by cells to maintain the availability of auxin. We report the first crystal structure of Wrightia tinctoria 11S globulin (WTG) in complex with Indole-3-acetic acid (IAA), an auxin, at 1.7 Å resolution. WTG hexamers assemble as a result of the stacking interaction between the hydrophobic surfaces of two trimers, leaving space for the binding of charged ligands. The bound auxin is stabilized by non-covalent interactions, contributed by four chains in each cavity. The presence of bound ligand was confirmed by matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) and high-resolution mass spectrometry (HRMS). Here, we hypothesize that the cleavage of globulins by endopeptidases leads to the movement of the hydrophilic loop region from the surface to the periphery, leaving space for the binding of auxin, and promotes hexamer formation. As the process of germination proceeds, there is a change in the pH, which induces the dissociation of the hexamer and the release of auxin. The compact hexameric assembly ensures the long-term, stable storage of the hormone. This suggests a role for globulin as a novel player in auxin homeostasis. © 2017 The Author(s).
Citation: Scientific Reports(2017), 7(1): -
URI: https://doi.org/10.1038/s41598-017-04518-7
http://repository.iitr.ac.in/handle/123456789/1010
Issue Date: 2017
Publisher: Nature Publishing Group
ISSN: 20452322
Author Scopus IDs: 57204102455
57218355625
36082537700
57194524446
57112522900
57193241611
57188847653
14628506400
57203506001
57214355701
12809587900
55064809000
Author Affiliations: Kumar, P., Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand, 247667, India
Kesari, P., Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand, 247667, India
Dhindwal, S., Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand, 247667, India
Choudhary, A.K., Department of Botany, University of Delhi, Delhi, 110007, India
Katiki, M., Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand, 247667, India
Neetu, Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand, 247667, India
Verma, A., Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand, 247667, India
Ambatipudi, K., Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand, 247667, India
Tomar, S., Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand, 247667, India
Sharma, A.K., Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand, 247667, India
Mishra, G., Department of Botany, University of Delhi, Delhi, 110007, India
Kumar, P., Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand, 247667, India
Corresponding Author: Kumar, P.; Department of Biotechnology, Indian Institute of Technology RoorkeeIndia; email: pravshai@gmail.com
Appears in Collections:Journal Publications [BT]

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